We are beginning the process of deriving molecular phenotypes for our knockout lines. The first stage has been to understand gene expression in wild-type embryos, to produce a temporal baseline expression reference. The baseline highlights patterns in the way that different genes are normally expressed as an embryo develops: when they are switched on and off; their abundance and whether their expression is covariant with other genes.
This dataset provides a control against which mutant transcriptome data can be compared, in order to understand the effect of individual gene knockouts on whole-embryo gene expression.
RNA-seq was used to establish the expression profiles for whole, wild-type mouse embryos of known sex at each somite number between 4 and 36 (excluding 29 – 33). This range corresponds roughly to the period E8.5 – E10.5, a vital period during which many organs and systems begin to develop.
The interpreted data is available in Expression Atlas as a temporal baseline expression reference.