DMDD Med23 report

Med23^em2Wtsi

gene synonyms

LINE VIABILITY: Lethal

SHOW LINE PHENOTYPES

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E9.5 -/- DMDD7556_placenta

Placenta

Ⓟ DMDD7556 data

E9.5 -/- DMDD7554_placenta

Placenta

Ⓟ DMDD7554 data

E9.5 -/- DMDD7551_placenta

Placenta

Ⓟ DMDD7551 data

E9.5 +/+ DMDD7555_placenta

Placenta

Ⓟ DMDD7555 data

E9.5 +/+ DMDD7553_placenta

Placenta

Ⓟ DMDD7553 data

E9.5 +/+ DMDD7552_placenta

Placenta

Ⓟ DMDD7552 data

HREM

Ⓟ PLACENTA PHENOTYPES

EMBRYO PHENOTYPES

SAMPLE INFORMATION

DMDD7556 E9.5

Single page view for DMDD7556

Med23^<em2Wtsi>/Med23^<em2Wtsi>

No HREM data is available for this embryo.

Placenta

ID	MP TERM
MP:0001716	abnormal placenta labyrinth morphology
MP:0002836	abnormal chorion morphology
MP:0005033	abnormal trophoblast giant cell morphology
MP:0008803	abnormal placental labyrinth vasculature morphology

Yolk sac

ID	MP TERM
MP:0002169	no abnormal phenotype detected

Images

IMAGE TYPE	PROBE/ANTIGEN PROBE/ANTIGEN E-Cadherin (Cdh1) was used to demarcate syncytiotrophoblast in the placental labyrinth at E9.5, and to label parietal trophoblast giant cells. Isolectin BSI-B4 was used to highlight the placental labyrinth and the decidua. Tpbpa was used to mark spongiotrophoblast and glycogen cells. Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers. ×	IMAGE
Hematoxylin and eosin	View placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Yolk sac immunofluorescence	Green: E-Cadherin Red: Laminin Blue: DAPIView yolk sac image at full resolution in a new window
Yolk sac immunofluorescence	Green: E-Cadherin Red: Laminin Blue: DAPIView yolk sac image at full resolution in a new window

PROPERTY	VALUE
Type	Mutant line
Viability of the line at P14	Lethal
Gene	Med23
Allele	Med23^<em2Wtsi>
Genotype	Med23^<em2Wtsi>/Med23^<em2Wtsi>
Strain	C57BL/6NTac
Stage	E9.5
Sex (morphological analysis)	Not determined
Sex (PCR analysis)	Female
DMDD ID	DMDD7556
Sanger ID	DABC25.1e
Order mouse	Order mouse from IMPC

DMDD7554 E9.5

Single page view for DMDD7554

Med23^<em2Wtsi>/Med23^<em2Wtsi>

No HREM data is available for this embryo.

Placenta

ID	MP TERM
MP:0002836	abnormal chorion morphology
MP:0008803	abnormal placental labyrinth vasculature morphology
MP:0005033	abnormal trophoblast giant cell morphology

Images

IMAGE TYPE	PROBE/ANTIGEN PROBE/ANTIGEN E-Cadherin (Cdh1) was used to demarcate syncytiotrophoblast in the placental labyrinth at E9.5, and to label parietal trophoblast giant cells. Isolectin BSI-B4 was used to highlight the placental labyrinth and the decidua. Tpbpa was used to mark spongiotrophoblast and glycogen cells. Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers. ×	IMAGE
Hematoxylin and eosin	View placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window

PROPERTY	VALUE
Type	Mutant line
Viability of the line at P14	Lethal
Gene	Med23
Allele	Med23^<em2Wtsi>
Genotype	Med23^<em2Wtsi>/Med23^<em2Wtsi>
Strain	C57BL/6NTac
Stage	E9.5
Sex (morphological analysis)	Not determined
Sex (PCR analysis)	Male
DMDD ID	DMDD7554
Sanger ID	DABC24.1c
Order mouse	Order mouse from IMPC

DMDD7551 E9.5

Single page view for DMDD7551

Med23^<em2Wtsi>/Med23^<em2Wtsi>

No HREM data is available for this embryo.

Placenta

ID	MP TERM
MP:0002836	abnormal chorion morphology
MP:0008803	abnormal placental labyrinth vasculature morphology
MP:0005033	abnormal trophoblast giant cell morphology

Images

IMAGE TYPE	PROBE/ANTIGEN PROBE/ANTIGEN E-Cadherin (Cdh1) was used to demarcate syncytiotrophoblast in the placental labyrinth at E9.5, and to label parietal trophoblast giant cells. Isolectin BSI-B4 was used to highlight the placental labyrinth and the decidua. Tpbpa was used to mark spongiotrophoblast and glycogen cells. Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers. ×	IMAGE
Hematoxylin and eosin	View placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window

PROPERTY	VALUE
Type	Mutant line
Viability of the line at P14	Lethal
Gene	Med23
Allele	Med23^<em2Wtsi>
Genotype	Med23^<em2Wtsi>/Med23^<em2Wtsi>
Strain	C57BL/6NTac
Stage	E9.5
Sex (morphological analysis)	Not determined
Sex (PCR analysis)	Not determined
Comments on sex	Potential sex reversal
DMDD ID	DMDD7551
Sanger ID	DABC30.1g
Order mouse	Order mouse from IMPC

DMDD7555 E9.5

Single page view for DMDD7555

+/+

No HREM data is available for this embryo.

Placenta

ID	MP TERM
MP:0002169	no abnormal phenotype detected

Images

IMAGE TYPE	PROBE/ANTIGEN PROBE/ANTIGEN E-Cadherin (Cdh1) was used to demarcate syncytiotrophoblast in the placental labyrinth at E9.5, and to label parietal trophoblast giant cells. Isolectin BSI-B4 was used to highlight the placental labyrinth and the decidua. Tpbpa was used to mark spongiotrophoblast and glycogen cells. Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers. ×	IMAGE
Hematoxylin and eosin	View placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window

PROPERTY	VALUE
Type	Mutant line
Viability of the line at P14	Lethal
Gene	Med23
Allele	Med23^<em2Wtsi>
Genotype	+/+
Strain	C57BL/6NTac
Stage	E9.5
Sex (morphological analysis)	Not determined
Sex (PCR analysis)	Male
DMDD ID	DMDD7555
Sanger ID	DABC25.1f
Order mouse	Order mouse from IMPC

DMDD7553 E9.5

Single page view for DMDD7553

+/+

No HREM data is available for this embryo.

Placenta

ID	MP TERM
MP:0002169	no abnormal phenotype detected

Yolk sac

ID	MP TERM
MP:0002169	no abnormal phenotype detected

Images

IMAGE TYPE	PROBE/ANTIGEN PROBE/ANTIGEN E-Cadherin (Cdh1) was used to demarcate syncytiotrophoblast in the placental labyrinth at E9.5, and to label parietal trophoblast giant cells. Isolectin BSI-B4 was used to highlight the placental labyrinth and the decidua. Tpbpa was used to mark spongiotrophoblast and glycogen cells. Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers. ×	IMAGE
Hematoxylin and eosin	View placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Yolk sac immunofluorescence	Green: E-Cadherin Red: Laminin Blue: DAPIView yolk sac image at full resolution in a new window
Yolk sac immunofluorescence	Green: E-Cadherin Red: Laminin Blue: DAPIView yolk sac image at full resolution in a new window

PROPERTY	VALUE
Type	Mutant line
Viability of the line at P14	Lethal
Gene	Med23
Allele	Med23^<em2Wtsi>
Genotype	+/+
Strain	C57BL/6NTac
Stage	E9.5
Sex (morphological analysis)	Not determined
Sex (PCR analysis)	Male
DMDD ID	DMDD7553
Sanger ID	DABC24.1e
Order mouse	Order mouse from IMPC

DMDD7552 E9.5

Single page view for DMDD7552

+/+

No HREM data is available for this embryo.

Placenta

ID	MP TERM
MP:0002169	no abnormal phenotype detected

Images

IMAGE TYPE	PROBE/ANTIGEN PROBE/ANTIGEN E-Cadherin (Cdh1) was used to demarcate syncytiotrophoblast in the placental labyrinth at E9.5, and to label parietal trophoblast giant cells. Isolectin BSI-B4 was used to highlight the placental labyrinth and the decidua. Tpbpa was used to mark spongiotrophoblast and glycogen cells. Yolk sacs were stained for E-Cadherin (green), Laminin (red) and DAPI. E-Cadherin labels the visceral yolk sac endoderm, Laminin labels the basement membrane between endoderm and mesoderm layers. ×	IMAGE
Hematoxylin and eosin	View placenta image at full resolution in a new window
In situ hybridisation	TpbpaView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window
Immunohistochemistry	E-CadherinView placenta image at full resolution in a new window

PROPERTY	VALUE
Type	Mutant line
Viability of the line at P14	Lethal
Gene	Med23
Allele	Med23^<em2Wtsi>
Genotype	+/+
Strain	C57BL/6NTac
Stage	E9.5
Sex (morphological analysis)	Not determined
Sex (PCR analysis)	Not determined
Comments on sex	Potential sex reversal
DMDD ID	DMDD7552
Sanger ID	DABC30.1c
Order mouse	Order mouse from IMPC

FIRST TIME HERE?

We recommend you take the 30 second page tour which provides an overview of the page functionality.
1/7 CAROUSEL

This shows all the embryos available for the line.
2/7 LINE SUMMARY

Phenotype data for the LINE can be viewed by clicking this button.
3/7 EMBRYO DATA

The lower part of the page shows all the data available for the sample highlighted in red.
4/7 DATA TABS

The tabs show different types of data collected for each sample.
5/7 PLACENTA PHENOTYPES ICONS

Availability of placental phenotype data is indicated by Ⓟ in the carousel above.
6/7 EMBRYO PHENOTYPES ICONS

Availability of embryo phenotype data is indicated by in the carousel above.
7/7 TOUR END

That's the end of the page tour. You can retake it anytime by clicking on the help icon.

30 SECOND PAGE TOUR

Provides an overview of line page functionality.
1/7 CAROUSEL

This shows all the embryos available for the line.
2/7 LINE SUMMARY

Phenotype data for the LINE can be viewed by clicking this button.
3/7 EMBRYO DATA

The lower part of the page shows all the data available for the sample highlighted in red.
4/7 DATA TABS

The tabs show different types of data collected for each sample.
5/7 PLACENTA PHENOTYPES ICON

Availability of placental phenotype data is indicated by Ⓟ in the carousel above.
6/7 EMBRYO PHENOTYPES ICON

Availability of embryo phenotype data is indicated by in the carousel above.
7/7 TOUR END

That's the end of the page tour. You can retake it anytime by clicking on the help icon.

Med23em2Wtsi

LINE VIABILITY: Lethal

PLACENTA PHENOTYPES (homozygous mutants)

E9.5 PLACENTA PHENOTYPES (homozygous mutants)

E14.5 PLACENTA PHENOTYPES (homozygous mutants)

EMBRYO PHENOTYPES (homozygous mutants)

E9.5 EMBRYO PHENOTYPES (homozygous mutants)

E14.5 EMBRYO PHENOTYPES (homozygous mutants)

DMDD7556 E9.5

Med23<em2Wtsi>/Med23<em2Wtsi>

Placenta

Yolk sac

Images

DMDD7554 E9.5

Med23<em2Wtsi>/Med23<em2Wtsi>

Placenta

Images

DMDD7551 E9.5

Med23<em2Wtsi>/Med23<em2Wtsi>

Placenta

Images

DMDD7555 E9.5

+/+

Placenta

Images

DMDD7553 E9.5

+/+

Placenta

Yolk sac

Images

DMDD7552 E9.5

+/+

Placenta

Images

FIRST TIME HERE?

1/7 CAROUSEL

2/7 LINE SUMMARY

3/7 EMBRYO DATA

4/7 DATA TABS

5/7 PLACENTA PHENOTYPES ICONS

6/7 EMBRYO PHENOTYPES ICONS

7/7 TOUR END

30 SECOND PAGE TOUR

1/7 CAROUSEL

2/7 LINE SUMMARY

3/7 EMBRYO DATA

4/7 DATA TABS

5/7 PLACENTA PHENOTYPES ICON

6/7 EMBRYO PHENOTYPES ICON

7/7 TOUR END

Med23^em2Wtsi

Med23^<em2Wtsi>/Med23^<em2Wtsi>

Med23^<em2Wtsi>/Med23^<em2Wtsi>

Med23^<em2Wtsi>/Med23^<em2Wtsi>